Journal: Materials

Article Title: Augmentation of DMLS Biomimetic Dental Implants with Weight-Bearing Strut to Balance of Biologic and Mechanical Demands: From Bench to Animal

doi: 10.3390/ma12010164

Figure Lengend Snippet: MG63 cells differentiation on DMLS Ti 6 Al 4 V discs: in vitro biocompatibility analysis. ( A ) MG63 cells attached, proliferated and differentiated well on both non-porous and porous DMLS Ti 6 Al 4 V discs. The MG63 cell clusters attached on the surface of discs and grew into the pores. Representative results of 10 independent experiments are shown. (Length of scale bar: as described) ( B ) Comparisons of alkaline phosphatase (ALP) mRNA gene expressions or ( C ) relative ALP activity after 14 days of culturing MG63 cells in control medium (non-differentiation), osteogenic (differentiation) medium, or on Ti 6 Al 4 V discs of various (0, 20, 40, 60 or 80%) porosities (n = 10). The ALP gene expression was significantly up-regulated in the titanium samples, which attained maximal expression at 60% porosity.

Article Snippet: Confluent MG63 (ATCC, Rockville, MD, USA) osteosarcoma cell line cultures were seeded on top of the sterilized discs.

Techniques: In Vitro, Activity Assay, Control, Gene Expression, Expressing

Journal: bioRxiv

Article Title: Proteome-wide multi-omics profiling of osteosarcoma transcription factor networks

doi: 10.64898/2026.03.29.714917

Figure Lengend Snippet: A) Schema of the concatenated tandem array of the consensus transcription factor response elements (catTFREs) workflow utilising two plasmids inserts C1 (designed with approximately 110 TF binding sites (TFBS) in duplicates with linkers) and C4 (reduced TFBS). Detailed TFBS are provided in Table S1A and B. Inserts were linearized by restriction enzyme digestion and biotinylated by Klenow enzyme. Biotinylated C1 and C4 were immobilized on streptavidin coated magnetic beads and incubated with nuclear extracts (NE) for detection of DNA binding proteins by mass spectrometry (MS). B) MS spectral counts of proteins enriched from NEs or by TFRE C1 and C4 pulldown. Four replicates with total count of proteins (left) and TFs only (right) from HOS (purple), U2OS (red) and MG63 (blue). All source data are provided in Table S1C-F. C) Heatmap representing the Pearson correlation of spectral counts by samples from HOS, U2OS and MG63. Scale represents low correlation in white to self-correlations as red-brown. D) Clustering of proteins by Weighted Gene Co-expression Network Analysis from MS spectral counts of NE and C1/C4 in HOS, MG63 and U2OS. Clusters I to X with the number of proteins represented by Pearson correlation across all conditions. Proteins are listed in Table S1G.

Article Snippet: Human osteosarcoma HOS, U2OS, and MG63 cell lines (CRL-1543, HTB-96 and CRL-1427, .) were purchased from the American Type Culture Collection (ATCC).

Techniques: Binding Assay, Magnetic Beads, Incubation, DNA Binding Assay, Mass Spectrometry, Expressing

Journal: bioRxiv

Article Title: Proteome-wide multi-omics profiling of osteosarcoma transcription factor networks

doi: 10.64898/2026.03.29.714917

Figure Lengend Snippet: A) Heat map of differential protein analysis between cell lines, C1, C4 and NE comparisons, defining clusters 1-6 (n = 4). B) Intensity levels of proteins JUNB and RUNX2 above; and TEAD1 and NFIB below, in NE and the catTFREs C1 and C4, for cell lines MG63, U2OS and HOS (n = 4). C, D, and E) Volcano plots of differential protein expression (DEP) analysis between C1 and NE in MG63 (in C), U2OS (in D), and HOS (in E), abs(log2FoldChange) > 1 and a p-value < 0.05. Significant proteins detected are coloured in blue (NE) and red (C1) with a selection of key proteins highlighted. All source data are provided in Table S2.

Article Snippet: Human osteosarcoma HOS, U2OS, and MG63 cell lines (CRL-1543, HTB-96 and CRL-1427, .) were purchased from the American Type Culture Collection (ATCC).

Techniques: Expressing, Selection

Journal: bioRxiv

Article Title: Proteome-wide multi-omics profiling of osteosarcoma transcription factor networks

doi: 10.64898/2026.03.29.714917

Figure Lengend Snippet: A) Volcano plots of the differential protein expression (DEPs) between catTFRE C1 for HOS (red) and MG63 (blue) cell lines. B) Volcano plots of the differential protein expression (DEPs) between catTFRE C1 for U2OS (red) and MG63 (blue) cell lines. C) Integration of the differential protein analysis for C1 from the HOS/MG63 and U2OS/MG63 comparison by log2Foldchanges and p-adjust values. Significant genes are clustered by log2FoldChange, highlighted by −log10(pValue HOS/MG63) and sized by −log10(pValue U2OS/MG63). D) Top 200 of the highest differential edge degrees between HOS/MG63, filtering based on the differential protein enrichment in A), plotted by Cytoscape. The edges in HOS and MG63 were highlighted in purple and green, respectively. E) Top 200 of the highest differential edge degrees between U2OS/MG63, filtering based on the differential protein enrichment in B). The edges in U2OS and MG63 were highlighted in purple and green, respectively. All source data are provided in Table S3.

Article Snippet: Human osteosarcoma HOS, U2OS, and MG63 cell lines (CRL-1543, HTB-96 and CRL-1427, .) were purchased from the American Type Culture Collection (ATCC).

Techniques: Expressing, Comparison, Protein Enrichment

Journal: bioRxiv

Article Title: Proteome-wide multi-omics profiling of osteosarcoma transcription factor networks

doi: 10.64898/2026.03.29.714917

Figure Lengend Snippet: A) and B) Differential gene expression (DEGs) in the comparison of U2OS/MG63 and HOS/MG63. Statistically significant higher expression of HOS and U2OS genes are highlighted in red, while blue indicates higher expression of MG63. D) and E) Integration of the logFoldChange of DEPs and DEGs of U2OS/MG63 (in A) and HOS/MG63 (in B), with |log2FoldChange catTFREs C1| > 1 and |log2FoldChange RNA-seq| > 1. Four clusters are indicated by colours red, blue, purple, and yellow. Each cluster has a different number of proteins and key genes annotated. E) A Venn diagram of integrated datasets of U2OS and HOS for catTFRE up and RNA-seq up. All source data are provided in Table S4.

Article Snippet: Human osteosarcoma HOS, U2OS, and MG63 cell lines (CRL-1543, HTB-96 and CRL-1427, .) were purchased from the American Type Culture Collection (ATCC).

Techniques: Gene Expression, Comparison, Expressing, RNA Sequencing

Journal: bioRxiv

Article Title: Proteome-wide multi-omics profiling of osteosarcoma transcription factor networks

doi: 10.64898/2026.03.29.714917

Figure Lengend Snippet: A) Heatmap visualizing the correlation of catTFREs C1 MS , RNA-seq , and consensus peaks at TSS (ATAC-seq DARs) that are highly correlated. All genes/proteins were filtered based on the Pearson correlation between each pair of omics, where at least one pair had a correlation coefficient greater than 0.5. Four clusters are annotated where A is specific to MG63, B specific to HOS/U2OS, C specific to HOS and D specific to U2OS. B) Overview of high confidence of the predicted protein–protein interactions using the StringDb database. The cluster represents the protein and its interaction greater than 0.4 from the HOS/U2OS-shared cluster (Cluster B). C) Heatmap visualizing the normalized gene expression RPKM of all the TFs enriched in footprinting analysis in MG63, HOS and U2OS that were significant in catTFRE C1 comparison in , and . Four replicates of RNA-seq per cell line. D) Ranking of TF footprinting from whole-genome consensus peaks in MG63 (left), HOS (middle) and U2OS (right) using chromVAR. TFs are coloured in blue (not significant), magenta (significant), and grey (not detected) for catTFREs C1 differential comparison from , and and integrated with RNA-seq, MG63/HOS or MG63/U2OS for each cell line. All source data are provided in Table S5.

Article Snippet: Human osteosarcoma HOS, U2OS, and MG63 cell lines (CRL-1543, HTB-96 and CRL-1427, .) were purchased from the American Type Culture Collection (ATCC).

Techniques: RNA Sequencing, Protein-Protein interactions, Gene Expression, Footprinting, Comparison

Journal: Oncology Reports

Article Title: Long non-coding RNA PCAT6 promotes the development of osteosarcoma by increasing MDM2 expression

doi: 10.3892/or.2020.7813

Figure Lengend Snippet: LncRNA PCAT6 promotes osteosarcoma cell proliferation. (A) LncRNA PCAT6 expression in different cells was determined *P<0.05 and ***P<0.001. MDM2 expression in different cells was detected using (B) RT-qPCR and (C) western blotting *P<0.05 and ***P<0.001. (D) LncRNA PCAT6 expression was determined using RT-qPCR following lncRNA PCAT6 expression ***P<0.001. (E) Cell Counting Kit-8 assays were performed to detect osteosarcoma cell proliferation following lncRNA PCAT6 overexpression. *P<0.05, **P<0.01 and ***P<0.001 vs. NC. lncRNA PCAT6, long non-coding RNA prostate cancer-associated transcript 6; MDM2, E3 ubiquitin-protein ligase Mdm2; RT-qPCR, reverse transcription-quantitative PCR; NC, negative control; OE, overexpression; OD, optical density.

Article Snippet: Normal osteoblasts (hFOB1.19) and human osteosarcoma cell lines (Saos2, MG63, U2OS and HOS) used in the present study were purchased from American Type Culture Collection.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Cell Counting, Over Expression, Ubiquitin Proteomics, Reverse Transcription, Real-time Polymerase Chain Reaction, Negative Control

Journal: Oncology Reports

Article Title: Long non-coding RNA PCAT6 promotes the development of osteosarcoma by increasing MDM2 expression

doi: 10.3892/or.2020.7813

Figure Lengend Snippet: LncRNA PCAT6 enhances the migration and invasion of osteosarcoma cells. (A) The migration and invasion of osteosarcoma cells increased following overexpression of lncRNA PCAT6. (B) MMP-2 and MMP-9 expression was detected using western blotting. *P<0.05, **P<0.01 and ***P<0.001. lncRNA PCAT6, long non-coding RNA prostate cancer-associated transcript 6; NC, negative control; OE, overexpression.

Article Snippet: Normal osteoblasts (hFOB1.19) and human osteosarcoma cell lines (Saos2, MG63, U2OS and HOS) used in the present study were purchased from American Type Culture Collection.

Techniques: Migration, Over Expression, Expressing, Western Blot, Negative Control

Journal: Oncology Reports

Article Title: Long non-coding RNA PCAT6 promotes the development of osteosarcoma by increasing MDM2 expression

doi: 10.3892/or.2020.7813

Figure Lengend Snippet: Overexpression of lncRNA PCAT6 promoted the proliferation of osteosarcoma cells in vivo . (A) The daily weight of mice was recorded, and the percentage of weight loss was calculated. (B) Tumor volume. (C) Tumor size. (D) Tumor diameter. (E) Tumor weight. (F) HE staining in osteosarcoma tissue was performed to analyze pathological characteristics and immunohistochemistry was performed to detect ki-67 expression. *P<0.05, **P<0.01 and ***P<0.001 vs. NC. lncRNA PCAT6, long non-coding RNA prostate cancer-associated transcript 6; NC, negative control; OE, overexpression; HE, hematoxylin-eosin.

Article Snippet: Normal osteoblasts (hFOB1.19) and human osteosarcoma cell lines (Saos2, MG63, U2OS and HOS) used in the present study were purchased from American Type Culture Collection.

Techniques: Over Expression, In Vivo, Staining, Immunohistochemistry, Expressing, Negative Control

Journal: Oncology Reports

Article Title: Long non-coding RNA PCAT6 promotes the development of osteosarcoma by increasing MDM2 expression

doi: 10.3892/or.2020.7813

Figure Lengend Snippet: Knockdown of MDM2 inhibits the proliferation, migration and invasion of osteosarcoma cells. (A) MDM2 expression was detected using western blotting. ***P<0.001. (B) Cell Counting Kit-8 assays were performed to detect osteosarcoma cell proliferation. *P<0.05, **P<0.01 and ***P<0.001 vs. OE PCAT6. (C) Wound healing and Transwell assays were performed to determine the migration and invasion of osteosarcoma cells following MDM2 knockdown, respectively. ***P<0.001. lncRNA PCAT6, long non-coding RNA prostate cancer-associated transcript 6; NC, negative control; OE, overexpression; MDM2, E3 ubiquitin-protein ligase Mdm2; si, small interfering RNA; OD, optical density.

Article Snippet: Normal osteoblasts (hFOB1.19) and human osteosarcoma cell lines (Saos2, MG63, U2OS and HOS) used in the present study were purchased from American Type Culture Collection.

Techniques: Knockdown, Migration, Expressing, Western Blot, Cell Counting, Negative Control, Over Expression, Ubiquitin Proteomics, Small Interfering RNA

Journal: Oncology Reports

Article Title: Long non-coding RNA PCAT6 promotes the development of osteosarcoma by increasing MDM2 expression

doi: 10.3892/or.2020.7813

Figure Lengend Snippet: Knockdown of MDM2 suppresses osteosarcoma cell proliferation in vivo . (A) Mice weight. (B) Daily tumor volume. *P<0.05, **P<0.01 and ***P<0.001, OE PCAT6 group vs. OE PCAT6 + MDM2 group. # P<0.05, ## P<0.01 and ### P<0.001 OE PCAT6 group vs. OE PCAT6 + RG7112 group. (C-E) Tumor weight. (G and F) HE staining in osteosarcoma tissue and immunohistochemistry was performed to determine ki-67 expression in tumor tissues. ***P<0.001. lncRNA PCAT6, long non-coding RNA prostate cancer-associated transcript 6; NC, negative control; OE, overexpression; MDM2, E3 ubiquitin-protein ligase Mdm2; si, small interfering RNA.

Article Snippet: Normal osteoblasts (hFOB1.19) and human osteosarcoma cell lines (Saos2, MG63, U2OS and HOS) used in the present study were purchased from American Type Culture Collection.

Techniques: Knockdown, In Vivo, Staining, Immunohistochemistry, Expressing, Negative Control, Over Expression, Ubiquitin Proteomics, Small Interfering RNA